Nonrandom F-plasmid replication in Escherichia coli K-12

Nonrandom F-plasmid replication in Escherichia coli K-12.

Both the autonomous and chromosomally integrated F plasmids were found to replicate in a nonrandom fashion after a density transfer from heavy medium ([13C]glucose, 15NH4) to light medium ([12C]glucose, 14NH4). The data are consistent with the hypothesis that both the chromosome and the F plasmid are replicated in a cell cycle-specific manner. Thus, these data support the proposal (J. D. Keasli...

Replication of the nonconjugative plasmid RSF1010 in Escherichia coli K-12.

Replicating DNA molecules of the nonconjugative R plasmid RSF1010 (Smr Sur) were cleaved with the EcoRI restriction endonuclease and examined with the electron microscope. Results of this analysis indicated that replication is initiated from an origin located at about 19% of total genome size from one of the EcoRI ends. Replication proceeded either unidirectionally or bidirectionally with equal...

Escherichia coli K-12 F-prime factors, old and new.

Requirements for replication restart proteins during constitutive stable DNA replication in Escherichia coli K-12.

Constitutive stable DNA replication (cSDR) is a mechanism for replisome loading in Escherichia coli K-12. This occurs in a dnaA-independent fashion in an rnhA mutant. cSDR is dependent on recA, priA, and transcription. In this report, it is shown that dnaA rnhA mutants using cSDR for initiation of their DNA replication additionally require priB, but not priC, for viability. Two subtle priA miss...

Transduction in Escherichia Coli K-12.

SYSTEM of genetic transduction has been discovered in the sexually fertile A K-12 strain of Escherichia coli. This transduction is mediated by lambda, a temperate phage for which K-12 is normally lysogenic. The distinctive features of the lambda-K-12 system include the following: (1) The transductions are limited to a cluster of genes for galactose fermentation. The GaZ loci are closely linked ...